Oak BioSciences offers a wide range of antibody engineering services that complement your research and accelerate the process from development to clinical use. The antibody engineering services listed below are offered as stand-alone services and also as part of a complete antibody humanization package. We can be your solution for antibody engineering – driving your research from any stage to final production of 100% royalty free humanized antibodies.

Antibody Cloning and Sequencing

Our antibody sequencing service is quick and reliable, and is offered as a stand-alone product or part of an antibody humanization package. With our satisfaction guarantee, you are assured to receive reliable results with a full report of the consensus variable fragments of heavy chain (VH) and light chain (VL), CDR and framework determination, and cDNA construct.

Rapid Custom Monoclonal Antibody (mAb) Hybridoma Production

Our antibody generation service employs a variety of technologies including hybridoma and library screening. Working closely with you, we provide on design and expression consultation on mAb engineering and manufacturing to meet your specific requirements. Each project is divided into three phases: antigen immunization and screening, fusion screening, and cloning and production. Typical project turnaround time is 8-12 weeks.

Antibody Isotype/Class/Subclass Switching

The antibody isotype switching process involves replacing only the constant region of an  antibody while maintaining the variable regions, thus preserving the specificity of your antibody. Antibody class switching does not affect antigen specificity, but retains antigen affinity and allows interaction with different effector molecules. Using our proprietary technology and in-house expertise, we are able to modify antibody’s isotype from any species to human IgG isotypes.

Recombinant Antibody (Chimeric Antibody) Construction and Production

Oak BioSciences has developed a proprietary technology for rapid production of cell lines expressing full length chimeric antibodies, allowing customers to receive antibodies designed and produced with specific expression and characterization requirements. Chimeric antibodies contain variable domains, sequenced from your hybridoma cell line, which can be combined with any constant domain.

Single-Chain Variable Fragment (scFv-Fc) Construction and Production

ScFv’s offer multiple advantages over monoclonal antibodies as drug carriers to tumors, such as, greater tumor penetration, low kidney uptake, rapid blood clearance, and less negative response from human immune system. Our service provides scFv construction via three different methods:

  • scFv Produced from Synthetic DNA
  • scFv Produced from Monoclonal Cell Line
  • scFv Manufactured from Accession Number

In-Vivo High Efficiency Biotinylated Single-Chain Variable Fragment (scFv) Production

Through the high specificity and affinity of Biotin-Streptavidin/Avidin interaction, biotinylated antibody fragments are of special interest due to their applicability and reliability in various automated protein assays. Using our proprietary techniques, we are able to obtain a scFv biotinylation rate of up to 100% in vivo.

Antibody Library Construction

Oak BioSciences specializes in the construction of various antibody libraries, including familiar scFv, Fab, VHH and other customized formats, using human plasma cells from healthy donors and patients, and splenocytes from either naïve or immunized mice, or other sources.

Phage Display Screening from Combinatorial Libraries

Our scientists have extensive experience in the screening of phage display libraries. We can achieve an affinity of 10-7~10-9 for scFv/Fab antibodies by conducting library screening and panning for 3-4 cycles.

Antibody Humanization

Oak BioSciences provides a proprietary antibody humanization service. Using our 3-D modelling and V-fusion Cloning technologies, the sequences of antibody variable domains, which determine its binding specificity, can be incorporated into human donor sequences, thus creating a panel of full length humanized antibodies and high affinity antibodies for your needs. Through panning our site-specific designed phage display libraries, we could enhance affinity from 10 to 1000 times.

Antibody Affinity Maturation Service 

Our in vitro affinity maturation service is designed for optimization of antibodies, antibody fragments or other peptide molecules, such as antibody mimetics. With a few rounds of mutation and selection, affinity for antibody fragments can increase 10-1,000 folds.

Stable Cell Line Development for Monoclonal Antibody (mAb) Production

Oak BioSciences can help you develop stable mammalian cell line from your monoclonal mouse cell lines or simply from a peptide/DNA sequence or accession number. We offer a choice of in-house or commercially available expression vectors.

Antibody Epitope Mapping

Phage libraries displaying millions of peptides with randomized sequences are extremely useful tools for mapping antibody epitopes. Oak Biosciences has created random peptide libraries by inserting short synthetic oligonucleotides with a completely randomized sequence usually at the engineered 5’-end of gIII gene.