B-cell Epitope Mapping (Antibody Epitope Mapping)

Oak Biosciences provides antibody epitope mapping using phage display peptide libraries.

Phage libraries displaying millions of peptides with randomized sequences are extremely useful tools for mapping antibody epitopes. Oak Biosciences has created random peptide libraries by inserting short synthetic oligonucleotides with a completely randomized sequence usually at the engineered 5’-end of gIII gene. Huge libraries with about 1010 individual phages, each containing a unique nucleotide sequence, have been generated. A single phage then provides a unique peptide sequence displayed as fusion to the engineered coat protein gIII.

In many cases, antibodies are able to select peptides with reasonable affinity for their combining sites (paratopes) from these libraries. Ideally, consensus motives can be deduced from multiple peptide sequences and matched to areas of the antigen against which the antibody was raised, and thus critical components of the antibody epitope can be defined.

The main procedure includes:

  1. Peptide Phage Display library preparation and functional confirmation
  2. Affinity Selection of Antibody-Binding Phage
  3. Monoclonal Phage ELISA
  4. Phage Sequencing
  5. Data Analysis
  6. Epitope Mapping Report

Please contact us for pricing details.