Antibody Phage Display Library Panning Services
Phage display is a laboratory technique that uses bacteriophages to associate proteins with their respective genetic information for studying protein–protein, protein–peptide, and protein–DNA interactions. The antibody phage display technique is based on genetic engineering of bacteriophages and repeated rounds of antigen-guided selection and phage propagation. It allows in vitro selection and production of recombinant monoclonal antibodies of high specificity and affinity.
Phage display-derived monoclonal antibodies have revolutionized drug discovery and represent an alternative technique to the traditional hybridoma technology. Oak Biosciences scientists have applied this technology to develop antibodies for therapeutic or diagnostic antibody applications.
Oak Biosciences has a wide range of ready-to-panning antibody phage libraries, including non-immune libraries, such as naive antibody phage library or synthetic antibody phage libraries, and immune antibody libraries from immunized animals or patients. Those ready-to-panning antibody phage libraries present various formats, such as scFv, Fab, VHH and affibody, to make it convenient for different target panning and for a variety of applications.
Our antibody phage display panning services allow the selection (“panning”) of recombinant antibody fragments from antibody gene libraries and screening of individually selected antibody clones with high specificity and affinity.
Oak Biosciences have different types and formats ready-to-panning phage libraries for your targets panning.
1. Antibody Phage Display Libraries (ScFv, Fab and VHH ready-to-panning phage libraries)
| Library Name | Format | Diversity (pfu/ml) checked by NGS | Comments |
|---|---|---|---|
| Human Naïve scFv Phage Display Library | VH-Linker-Vκ | 6.0×109 | Library was constructed from different ethnic groups (Asia, African American, Caucasian, Filipino, Hispanic, and each groups has at least 50 donors aged from 25 to 74 with equally distributed male and female. |
| Human Naïve Fab Phage Display Library | Vκ,λ-Cκ,λ//VH-CH1 | 6.0×109 | Library was constructed from different ethnic groups (Asia, African American, Caucasian, Filipino, Hispanic, and each groups has at least 50 donors aged from 25 to 74 with equally distributed male and female. |
| Human Synthetic scFv Phage Display Library | VH-Linker-Vκ | 4.0×109 | Framework: IGKV1 subgroup & IGHV1 family |
| Human Synthetic Fab Phage Display Library | Vκ-Cκ//VH-CH1 | 3.0×109 | Framework: IGKV4 subgroup & IGHV1 family |
| Murine Synthetic scFv Phage Display Library | VH-Linker-Vκ | 4.0×109 | Framework: IGKV1 subgroup & IGHV1 family |
| Murine Synthetic Fab Phage Display Library | Vκ-Cκ//VH-CH1 | 4.0×109 | Framework: IGKV4 subgroup & IGHV1 family |
| Murine Naïve scFv Phage Display Library | Vκ-Linker-VH | 1.8×109 | Library was constructed from spleens of BALB/c female mice (6–8 weeks old) |
| Rabbit Naïve scFv Phage Display Library | Vκ-Linker-VH | 2.1×1010 | Library was constructed from spleens of NZW SPF rabbits |
| Chicken Naïve scFv Phage Display Library | VH-Linker-Vκ | 1.6×109 | Library was constructed from spleens of white Leghorn hens (12 weeks old) |
| Synthetic VHH /SdAb Phage Display Library | VHH with HA tag | 5.1x109 | Framework: ADE99145 |
2. Peptide Phage Display Libraries (Cyclized C8C and linearized X8, X16 and X24 peptide phage display libraries)
Oak Biosciences peptide phage display system is based on a simple M13 phage vector as N-terminal fusions to the minor coat protein pIII. The peptide phage display libraries have been used for various applications, including epitope mapping, anti-microbial/viral peptides, material-specific peptides, bioactive peptides, small molecule binders, cell targeting peptides and novel enzyme substrates discovery through in vitro and in vivo panning approaches.
For Special application using our peptide phage display system for peptide aptamer development, please see the linker PEPTIDE APTAMER DEVELOPMENT SERVICES!
Combinatorial Peptide Libraries
Library Description Format Diversity/Phage titer (pfu/ml) checked by NGS Comments
C8C Loop-constrained octapeptide peptide phage display library OmpA leader-Csyteine-Octapeptide-Cysteine-6X His tag-HA tag-Amber stop codon 7.4x109/7.4X1011 Randomized cyclized 8-amino acid peptide with 6xHis Tag and HA Tag
X8 Linear 8-amino acid peptide phage display library Randomized 8-amino acid peptide with 6xHis Tag and HA Tag (OmpA leader sequence- X8 peptide -6xHis tag-HA tag- Amber stop codon) 7.9×109/7.9X1011 Randomized 8-amino acid peptide with 6xHis Tag and HA Tag
X16 Linear 16-amino acid peptide phage display library Randomized 16-amino acid peptide with 6xHis Tag and HA Tag (OmpA leader sequence- X16 peptide-6xHis tag-HA tag-Amber stop codon) 8.6×109/8.6X1011 Randomized 16-amino acid peptide with 6xHis Tag and HA Tag
X24 Linear 24-amino acid peptide phage display library Randomized 16-amino acid peptide with 6xHis Tag and HA Tag (OmpA leader sequence- X16 peptide-6xHis tag-HA tag-Amber stop codon) 8.2×109/8.2X1111 Randomized 16-amino acid peptide with 6xHis Tag and HA Tag
3. Affibody ready-to-panning phage display library
Affibody molecules are a class of small robust scaffold proteins derived from the IgG binding domain of Staphylococcus aureus protein A (SPA). Thirteen specific amino acids in the three α-helix regions of the IgG binding domain can be randomly mutated to construct an affibody library. This library can be screened to obtain affibody molecules with high affinity and specificity to any given target molecule.
For Special application using our Affibody phage display system for Affibody development, please see the linker Affibody DEVELOPMENT SERVICES!
Z-affibody Library
Library Description Format Diversity/Phage titer (pfu/ml) checked by NGS
Z-affibody Constructed by saturation mutagenesis of the Z domain with random amino acid residues at positions 9, 10, 11, 13, 14, 17, 18, 24, 25, 27, 28, 32 and 35. OmpA leader sequence-Z-affibody 4.8×109/4.8×1011
Deliverables
a) Panning raw data
b) ELISA validated candidates (3-24 unique clones, sequences and plasmids)
c) Detail project report
Ordering Information
Antibody Phage Display Library Panning and Candidate Validation Services
| Catalog | Description | Unit | Price |
|---|---|---|---|
| 88101 | phage display library panning and candidate scFv validation (scFv Format) | 1 Project | Contact Us |
| 88102 | phage display library panning and candidate Fab validation (Fab Format) | 1 Project | Contact Us |
| 88103 | phage display library panning and candidate VHH validation (VHH Format) | 1 Project | Contact Us |
| 88104 | phage display peptide library panning and validation (C8C, X8, X16 and X24 peptide Format) | 1 Project | Contact Us |
| 88105 | phage display library panning and candidate affibody validation (Z-affibody Format) | 1 Project | Contact Us |
Some Citations Used Oak Biosciences Ready-to-Panning Libraries
1.Kristopher A Lofgren, Sreeja Sreekumar, E Charles Jenkins Jr, Kyle J Ernzen, Paraic A Kenny, Anti-tumor efficacy of an MMAE-conjugated antibody targeting cell surface TACE/ADAM17-cleaved Amphiregulin in breast cancer, Antibody Therapeutics, Volume 4, Issue 4, October 2021, Pages 252–261, https://doi.org/10.1093/abt/tbab026 (The AREG antibodies were obtained from Oak Biosciences naïve rabbit scFv library and could not get specific binding monoclonal antibodies using Bio-Rad HuCAL® Library)
2. ShaniZilberman-Itskovich, RamziaAbu-Hamad, Moshe Stark & Shai Efrati (2019) Effect of anti-C5 antibody on recuperation from ischemia/reperfusion-induced acute kidney injury, Renal Failure, 41:1, 967-975, https://doi.org/ 10.1080/0886022X.2019.1677248 (The anti-C5 antibody was from Oak Biosciences human naïve Fab library)
3.Lenka Kyrych Sadilkova, Iva Valentova, Simona Hoskova, Pavel Vopalensky, Lukas Bammert, Roger Beerli, Ulrich Moebius, Radek Spisek. SO-N102, a novel CLDN18.2-targeting antibody-drug conjugate with strong anti-tumor effect in various solid tumors expressing low target levels. Cancer Res (2021) 81 (13_Supplement): 1204. https://doi.org/10.1158/1538-7445.AM2021-1204 (The anti-CLDN18.2 antibody was from Oak Biosciences chicken scFv library and then humanization by Oak Biosciences)
4.Chen Y, Jia M, Wang S, Xu S and He N (2022) Antagonistic Antibody Targeting TNFR2 Inhibits Regulatory T Cell Function to Promote Anti-Tumor Activity. Front. Immunol. 13:835690. https://doi.org/10.3389/fimmu.2022.835690 (The anti-TNFR2 antibody was from Oak Biosciences Rabbit Immunized scFv library and then humanization by Oak Biosciences)
For detailed pricing information, please fill the form below for a customized quotation!