Custom CRISPR/Cas9-based Genome Engineering
A significant recent advance in genome engineering is the development of the CRISPR/Cas9-based genome editing system. In this system, three components (crRNA, tracrRNA and CRISPR-associated Cas9 protein) are needed for genome editing in vivo. We could fuse the crRNA and tracrRNA into a single guide RNA (sgRNA) and induce efficient Cas9 cleavage in vitro. The sgRNA can be targeted to any desired genomic sequence through complementary base pairing with high levels of efficacy and specificity.
Use CRISPR/Cas9 technology to:
• Insert gene(s) into any specific genomic loci
• Knock-out expression of gene(s) in any specific genomic loci
• Varied systems in mammalian genomes, Escherichia coli and Yeast
Oak Biosciences’ CRISPR/Cas9 system offers proven technology with unparalleled support and expertise to make your use of this system easy and successful from the start.
Oak Biosciences provides:
- Customized CRISPR sgRNA and donor plasmids design and construction services
- Custom knock-in (KI) /knock-out (KO) cell line generation services
- gene point mutation, gene replacement, gene tagging, gene deletion and small DNA insertion services
- Guide RNA Vector Evaluation /Validation services